1. PRODUCT DESCRIPTION

Product Name‌: Pullulanase (EC 3.2.1.41)

Synonyms‌: Amylopectin 6-glucanohydrolase; Limit dextrinase

CAS No.‌: 9075-68-7

Appearance‌: White to light brown powder

Source‌: Microbial fermentation

Core Functions

α-1,6-Glycosidic Bond Hydrolysis‌: Cleaves branch points in amylopectin/glycogen to produce linear dextrins. Short-Chain Specificity‌: Degrades side chains as short as maltotriose (2-3 glucose units).

‌2. PRODUCT ADVANTAGE

Key Features‌:

Precision Debranching‌: Specifically hydrolyzes α-1,6-glycosidic bonds in starch/pullulan with higher efficiency than isoamylase.

Process Optimization‌: Reduces saccharification time by 8-16 hours when combined with glucoamylase (optimal ratio 1:2).

Yield Enhancement‌: Increases glucose/maltose yield to 96.5% DE and 70% maltose content respectively.

Acid-Thermostable‌: Maintains activity at pH 4.0-6.0 and 55-60°C, compatible with industrial conditions.

Multi-Industry Application‌: Food (syrups/beer), feed, cosmetics, and pharmaceutical excipient production.

‌3. TECHNICAL PARAMETERS/MECHANISM

Mechanism of Action：

 As an ‌α-1,6-glucanase‌, pullulanase:

Recognizes branch points via conserved Asp/Glu residues.

Hydrolyzes α-1,6 linkages in pullulan (linear α-1,6-linked maltotriose polymer), then amylopectin/glycogen.

Releases linear oligosaccharides for further digestion by other amylases.

Industrial Applications：

Food Industry

Application                  Benefit                                       Technical Outcome

Glucose Syrup‌                  +5%                       yield DE >96.5% with reduced oligosaccharides

Maltose Syrup‌            70% content                        Lower branched oligosaccharides

Beer Brewing‌            75%+ fermentability           Reduced dextrins in dry beer production

Other Sectors

Feed Additive‌:   improved feed conversion ratio in poultry

Pharmaceuticals‌:  e.g., maltosyl-cyclodextrins

Usage Recommendations：

Dosage‌: 0.1-0.3% of substrate (adjust by enzyme activity)

Optimal Conditions‌: pH 4.5-5.0, 55-60°C, 8h reaction

4.PACKAGE AND STORAGE

• Package：25kg/drum.
• Storage：Keep sealed in a dry and cool place and avoid direct sunlight. Slight sedimentation is acceptable since it will not impact performance of the product.
• Shelf life: 12 months in a dry and cool place.